Journal: Free radical biology & medicine

Article Title: Ultrasound sensitive O 2 microbubbles radiosensitize murine breast cancer but lead to higher metastatic spread.

doi: 10.1016/j.freeradbiomed.2023.02.022

Figure Lengend Snippet: Fig. 6. Impact of oxygen microbubbles on the level of β-catenin and N-cadherin in tumor homogenates collected during dissection of mice from the experimental groups described. A) Results of the densitometric analysis normalized to β-actin. B) Representative Western Blot results for the proteins described. N = 3 in each group, total N = 18. The error bars represent SEMs for the data of each group.

Article Snippet: The protein was then transferred to a nitrocellulose membrane, blocked with PBS-T solution (PBS with 0.1% Tween-20) solution containing 5% nonfat milk, and incubated overnight at 4◦C with a primary antibody against β-catenin and N-cadherin (D10A8, D4R1H, 1:1000, Cell Signaling, USA).

Techniques: Dissection, Western Blot

Journal: British Journal of Cancer

Article Title: Targeting pancreatic and ovarian carcinomas using the auristatin-based anti-CD70 antibody–drug conjugate SGN-75

doi: 10.1038/sj.bjc.6605816

Figure Lengend Snippet: Characterisation of anti-CD70 1C1 and 5D12. ( A ) Western blots containing FLAG-tagged CD70 ECD (lanes 1 and 5) and membrane extracts from HEK 293 cells (lanes 2 and 6), HEK 293:CD70 transfectants (lanes 3 and 7) and 786-O cells (lanes 4 and 8) were reacted with mAb 1C1 (left panel) or mAb 5D12 (right panel). The mAb 1C1 and 5D12 specifically bound recombinant CD70 (lane 1 and 4) and three distinct bands ( * ) from CD70+ membrane extracts (lanes 3, 4, 7, and 8). Extracts from parental HEK 293 cells were negative as expected (lane 2 and 6). The β -actin control shows comparable quantities of protein were loaded for each membrane extract (lower panels). ( B ) IHC analysis on FFPE normal human tissues showing limited expression of CD70 in lymphoid tissues. Red stain indicates presence of CD70+ cells. 1C1 mAb was used for these set of images. × 400 magnification, scale bar=50 μ m.

Article Snippet: For western blot analysis, membranes were first blocked in PBS-T+1% BSA and then incubated with 0.5 μ g ml −1 of purified SG-21.1C1 or SG-21.5D12 in PBS-T+1% BSA at RT for 1 h. A rabbit polyclonal antibody against β -actin (Cell Signaling, Danvers, MA, USA) was used as protein loading control.

Techniques: Western Blot, Membrane, Recombinant, Control, Expressing, Staining

Journal: European Journal of Histochemistry : EJH

Article Title: Apoptosis pattern and alterations of expression of apoptosis-related factors of supporting cells in Kölliker’s organ in vivo in early stage after birth in rats

doi: 10.4081/ejh.2017.2706

Figure Lengend Snippet: Western blot analysis of bcl-2, caspase-3, caspase-8 and caspase-9 in Kölliker’s organ in vivo . Western blot analysis showed cytoplasmic proteins from the cochlear basilar membrane in P1, P3, P5, P7, P10, P12 and P14. Expression of proteins of Kölliker’s organ at different time points during 1-14 days in vivo ; a representative image of Western blots, which were performed using primary antibodies against bcl-2, caspase-3, caspase-8 and caspase-9 from each group. β-actin was used as an internal loading control.

Article Snippet: The blots were blocked with 5% nonfat dry milk in PBS-0.01% Tween 20 (PBS-T) for 1 h at room temperature and then probed with antibodies against β-actin (Protein tech, 1:2000), bcl-2, caspase-3, caspase-8, caspase-9 and cleaved caspase-3 (Cell Signaling, Danvers, MA, USA; 1:1000) for 1 h at room temperature and then overnight at 4°C.

Techniques: Western Blot, In Vivo, Membrane, Expressing, Control

Journal: Microorganisms

Article Title: The Distribution of Neospora caninum Secretory Proteins in Mouse and Calf Brains

doi: 10.3390/microorganisms13091970

Figure Lengend Snippet: The distribution of Neospora caninum proteins, NcSAG1, NcCYP, NcPF, NcGRA6 and NcGRA7, in HFF cells infected with N. caninum , analyzed by immunofluorescence. The upper images are the same as the lower ones but with increased green-channel intensity. NcSAG1, NcCYP, and NcPF were localized in the tachyzoites. NcGRA6 and NcGRA7 (green) were localized in the tachyzoites, the parasitophorous vacuole space, and the host cytoplasm. Red: anti-β tubulin; blue: DAPI. Bar = 20 µm.

Article Snippet: After being washed three times with PBST, the samples were incubated with mouse monoclonal antibody against β tubulin (clone: 1D4A4; Proteintech Group, Inc., Rosemont, IL, USA) for 1 h at 37 °C.

Techniques: Infection, Immunofluorescence